Monoclonal Antibody Preparation Against Nucleoprotein of Avian Influenza Virus Subtype H9N2

Production of monoclonal antibodies against recombinant nucleoprotein of avian influenza virus, serotype H9N2

Background: Avian influenza viruses (AIVs) including the subtype H9N2 cause considerable financial losses to poultry industries. Rapid and accurate diagnosis of avian influenza (AI) infection is important in control and eradication programs. OBJECTIVES: The aim of this study was to produce monoclonal antibodies (MAbs) specific for the nucleocapsid protein )NP (of AIV H9N2 subtype to improve dia...

Isolation and Identification of Avian Influenza Virus H9N2 Subtype

Preparation of Neuraminidase-Specific Antiserum from the H9N2 Subtype of Avian Influenza Virus

Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs. The harvested suspension was concentrated by polyethylene glycol 6000. Concentrated samples were layered onto sucrose gradient (30%-60%). Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100 across 30% sucrose gr...

Preparation of anti-idiotypic antibody against avian influenza virus subtype H9.

To generate monoclonal anti-idiotypic antibodies (mAb2) against avian influenza virus subtype H9 (H9 AIV), BALB/c mice were immunized with purified chicken anti-H9-AIV IgG and the splenocytes of immunized mice were fused with myeloma cells NS-1. Hybridoma cells were screened by indirect enzyme-linked immunosorbent assays with both chicken and rabbit anti-H9-AIV IgG as coating antigens. One hybr...

isolation and identification of avian influenza virus h9n2 subtype

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